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nested primers中文什么意思

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In order to identifiy the virus further , a set of double nested primers for canine coronavirus was selected . the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl , ccvf2 - ccvr2 . the first is a pair of outer primer , and can amplify a fragement of 1086bp . the second is a pair of inner primer . and can amplify a fragement of 515bp . using the nested primers , many ccv strains can be identificated including k378 , insave - l , ccv 1 - 71 etc . synthesizing this set of primers , we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr , and all of them respectively gained two target fragements of 1086bp and 515bp , but the control cell did not
合成該套式引物，選擇大熊貓?jiān)×虾筒《靖鞔?xì)胞培養(yǎng)物，經(jīng)套式( nested ) rt一pcr擴(kuò)增，可得到一與設(shè)計(jì)值5巧bp相符的dna片段，經(jīng)bst一xl ( 590 ， 1110 )酶切鑒定，證明該擴(kuò)增片斷為特異性片段;回收大熊貓肝組織原代病料和細(xì)胞培養(yǎng)物第2 、 3 、 29代的ccvfz一ccvrz擴(kuò)增片段，純化，送生物公司測(cè)序。